Gas chromatographic analysis of malonaldehyde and 4‐hydroxy‐2‐(E)‐nonenal produced from arachidonic acid and linoleic acid in a lipid peroxidation model system | Zendy

Tamura H. | Zendy; Shibamoto T. | Zendy

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Gas chromatographic analysis of malonaldehyde and 4‐hydroxy‐2‐(E)‐nonenal produced from arachidonic acid and linoleic acid in a lipid peroxidation model system

Author(s) -

Tamura H.,

Shibamoto T.

Publication year - 1991

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02544014

Subject(s) - chemistry , arachidonic acid , linoleic acid , chromatography , gas chromatography , fatty acid , organic chemistry , enzyme

Malonaldehyde (MA) and 4‐hydroxynonenal (4‐HN) formed upon oxidation with Fe 2+ /H 2 O 2 from arachidonic acid and linoleic acid, and their ethyl esters were analyzed by gas chromatography (GC). The MA and 4‐HN produced were reacted with N ‐methylhydrazine (NMH) to give 1‐methylpyrazole and 5(1′‐hydroxyhexyl)‐1‐methyl‐2‐pyrazoline, respectively. The derivatives were analyzed by GC on a fused silica capillary column using a nitrogen‐phosphorus detector. With arachidonic acid, more MA and 4‐HN were formed from the ester (88 nmol/mg and 23 nmol/mg, respectively) than from the free acid (25 nmol/mg and 9 nmol/mg, respectively). In contrast, with linoleic acid, more MA and 4‐HN were produced from the free acid (53 nmol/mg and 13 nmol/mg, respectively) than from the ester (39 nm/mg and 8 nmol/mg, respectively).

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