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Platelet‐activating factor stimulates expression of IL‐1 beta mRNA in THP‐1 cells
Author(s) -
Barthelson Roger A.,
Valone Frank H.
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02543983
Subject(s) - thp1 cell line , messenger rna , platelet activating factor , beta (programming language) , microbiology and biotechnology , gene expression , lipidology , biology , rna , cell culture , clinical chemistry , chemistry , endocrinology , medicine , gene , biochemistry , genetics , computer science , programming language
Abstract A human monocytic cell line (THP‐1) was used to study the effects of PAF (platelet‐activating factor) on the expression of IL‐1 beta mRNA. THP‐1 cells were incubated with 10 pM PAF in the presence or absence of 0.1 μg/mL endotoxin for 4 hr, after which cytoplasmic RNA was extracted and subjected to Northern hybridizations. PAF, alone and in combination with endotoxin, caused an increase in mRNA levels for IL‐1 beta. The magnitude of the effects of PAF on IL‐1 beta mRNA levels matched closely the effects seen at the level of protein synthesis, suggesting that the effects of PAF on IL‐1 beta release may result largely from its effects on IL‐1 beta mRNA levels.