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Micro method for stereospecific analysis of triacyl‐ sn ‐glycerols by chiral‐phase high‐performance liquid chromatography
Author(s) -
Ando Yasuhiro,
Takagi Toru
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02543036
Subject(s) - chromatography , chemistry , stereospecificity , high performance liquid chromatography , magnesium bromide , column chromatography , bromide , chiral column chromatography , silica gel , hydrolysis , fatty acid ester , fatty acid , magnesium , organic chemistry , catalysis
Abstract A procedure for micro stereospecific analysis of triacyl‐ sn ‐glycerols (TGs) by high‐performance liquid chromatography (HPLC) on a chiral column is presented. TGs were partially hydrolyzed with ethyl magnesium bromide, and total products were immediately converted to 3,5‐dinitro‐phenylurethane derivatives. Each of the 1‐ and 2‐monoacylglycerol (MG) derivatives was isolated by HPLC on a silica column. The 1‐MGs were resolved into sn ‐1 and sn ‐3 MG fractions by HPLC on a Sumichiral OA‐4100 column (Sumitomo Chemical, Osaka, Japan). Fatty acid methyl esters obtained from the sn ‐1, sn ‐2 and sn ‐3 MG fractions were analyzed by gas‐liquid chromatography on a capillary column. Analyses of standard TGs showed that, even with 1 mg of sample, accuracy was comparable to that obtained with 100‐mg samples. Applying this procedure to the stereospecific analysis of 5 mg of jujube pulp, TGs revealed the positional distribution of the (n‐5) series of monounsaturated fatty acids they contained.