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Enzymatic synthesis of trierucin from high‐erucic acid rapeseed oil
Author(s) -
Trani Michael,
Lortie Robert,
Ergan Françoise
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02543021
Subject(s) - erucic acid , lipase , rapeseed , candida rugosa , interesterified fat , chemistry , hydrolysis , triacylglycerol lipase , chromatography , fatty acid , yield (engineering) , rhizopus arrhizus , food science , biochemistry , enzyme , materials science , metallurgy
The lipase from Candida rugosa has been shown to discriminate against erucic acid. Advantage of this property has been taken to produce trierucin from high‐erucic acid rapeseed (HEAR) oil. A method has been developed for extracting erucic acid from the oil as dierucin and subsequently enzymatically converting it to trierucin. Unrefined HEAR oil was hydrolyzed with lipase from C. rugosa to produce a mixture of free fatty acids and dierucin. Precipitation and filtration from cold ethanol gave 73% pure dierucin, free of fatty acids. This dierucin was treated in two ways to produce trierucin. First, in the presence of an immobilized lipase and a known amount of water, some trierucin is produced by interesterification. Second, a more efficient route to trierucin utilized Rhizopus arrhizus lipase to completely hydrolyze dierucin to erucic acid, which was then combined with an appropriate amount of dierucin in the presence of an immobilized lipase to produce trierucin in a quantitative yield.