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Effect of low‐dose γ‐radiation on individual phospholipids in aqueous suspension
Author(s) -
Tinsley P. W.,
Maerker G.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542624
Subject(s) - phosphatidic acid , chromatography , chemistry , dipalmitoylphosphatidylcholine , aqueous solution , phospholipid , aqueous suspension , irradiation , suspension (topology) , radiolysis , mass spectrometry , phosphatidylcholine , organic chemistry , biochemistry , membrane , physics , mathematics , homotopy , nuclear physics , pure mathematics
A series of individual phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines and phosphatidylglycerols) containing either saturated or unsaturated fatty acid chains was irradiated at 9.66 kGy and 0–4°C in aqueous suspension. The phospholipids were analyzed by normal‐phase high‐performance liquid chromatography on a silica column with an evaporative light scattering detector. Phospholipid disappearance and production of two radiolytic products, phosphatidic acid and the lysophospholipid, after irradiation were quantitated from calibration curves of synthetic standards. Dipalmitoylphosphatidic acid and monopalmitoylphosphatidylcholine from irradiated dipalmitoylphosphatidylcholine were identified by liquid secondary‐ion mass spectrometry.