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Inhibition of malonaldehyde formation from lipids by an isoflavonoid isolated from young green barley leaves
Author(s) -
Nishiyama T.,
Hagiwara Y.,
Hagiwara H.,
Shibamoto T.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542607
Subject(s) - chemistry , linolenate , butylated hydroxytoluene , ethyl diazoacetate , tocopherol , antioxidant , organic chemistry , nuclear chemistry , fatty acid , vitamin e , cyclopropanation , catalysis
The inhibitory effect of 2″‐O‐glycosylisovitexin (2″‐O‐GIV), isolated from young green barley leaves, on malonaldehyde (MA) formation from lipids was determined by gas chromatography. Two μmol of 2″‐O‐GIV inhibited MA formation from 0.2 mmol of squalene by almost 100% upon ultraviolet (UV) irradiation. Only 1 μmol of 2″‐O‐GIV inhibited 90% of MA formation from 0.2 mmol of ethyl linoleate upon UV irradiation. The effective quantities of 2″‐O‐GIV were much lower than those of either butylated hydroxytoluene or α‐tocopherol. When ethyl linoleate, ethyl linolenate and ethyl arachidonate were oxidized by Fenton’s reagent in the presence of 2″‐O‐GIV or α‐tocopherol, both compounds showed similar activity toward MA formation. Both compounds gave maximum inhibition at doses of 0.1–0.3 μmol for ethyl linoleate, 0.1–0.5 μmol for ethyl linolenate and 0.1–0.5 μmol for ethyl arachidonate.