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Determination of 6‐ketocholestanol in unirradiated and irradiated chicken meats
Author(s) -
Hwang K. T.,
Maerker G.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542602
Subject(s) - chemistry , chromatography , chloroform , irradiation , extraction (chemistry) , methanol , adsorption , gas chromatography , radiochemistry , organic chemistry , physics , nuclear physics
A method to detect 6‐ketocholestanol in unirradiated and irradiated chicken meats was developed by means of chloroform‐methanol‐water extraction, adsorption chromatographic column separation and gas chromatography. This method is able to measure cholesterol oxidation products at levels that are much lower than those of previous methods. The new procedure was used to detect 6‐ketocholestanol in fresh, unirradiated chicken and measured more than 97% of the test compound added to chicken below the ppm level. Irradiation of the chicken meats to a dose of 10 kGy increased the concentration of this compound to about four times the level of unirradiated meats.