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Conversion of oleic acid to 10‐ketostearic acid by a Staphylococcus species
Author(s) -
Lanser A. C.
Publication year - 1993
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542591
Subject(s) - oleic acid , bioconversion , fermentation , chemistry , microorganism , fatty acid , bacteria , food science , organic chemistry , chromatography , biochemistry , biology , genetics
In the course of using microorganisms to produce value‐added products from soybean oil or its fatty acids, a culture contaminant was isolated and tentatively identified as a Staphylococcus species. This microorganism converts oleic acid ( cis ‐9‐octadecenoic acid) to 10‐ketostearic acid (10‐keto‐octadecanoic acid) in growing cultures. Bioconversion was studied in two different media at temperatures from 30 to 41°C, with shaking at 150 rpm. Cells were grown in 50 mL of media for 24 h and then incubated with 0.25 g oleic acid for 24 h. Optimum conditions have allowed better than 90% conversion with 85% recovery. Unreacted oleic acid and 10‐hydroxystearic acid (10‐hydroxyoctadecanoic acid) are the only other compounds present in the ethyl ether extract of the fermentation broth.