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Modification of arachin‐effect of citrate ions‐structural implications
Author(s) -
Prakash V.,
Rao A. G. Appu,
Rao D. Rajagopal
Publication year - 1987
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542511
Subject(s) - chemistry , circular dichroism , dissociation (chemistry) , ion , dichroic glass , ultraviolet , citric acid , viscosity , crystallography , biophysics , biochemistry , organic chemistry , materials science , nanotechnology , optoelectronics , composite material , biology
The effect of the anion citrate on the subunit structure of arachin has been monitored by velocity sedimentation, viscosity and circular dichroism techniques. The results indicate that as the concentration of citrate ion increases from 25 mM to 150 mM, arachin dissociates progressively to the low molecular weight 2S component. This dissociation is not accompanied by any conformational changes or denaturation as revealed by the far ultraviolet circular dichroic spectra and viscosity results, respectively. The results are explained by direct anion binding and changes in the solvent structure.