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Analysis of conjugated octadecatrienoic acids in momordica balsamina seed oil by GLC and 13 C NMR Spectroscopy
Author(s) -
Gaydou E. M.,
Miralles J.,
Rasoazanakolona V.
Publication year - 1987
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542436
Subject(s) - conjugated system , chemistry , isomerization , organic chemistry , nuclear magnetic resonance spectroscopy , chromatography , stereochemistry , polymer , catalysis
Separation of conjugated octadecatrienoic acids by open tubular gas liquid chromatography (GLC) was performed using glass capillary columns coated with Carbowax 20 M and with OV‐1. The equivalent chain length of geometrical isomers of the conjugated octadecatrienoic acids belonging to the two series C 18:3 Δ 8.10.12 and C 18:3 Δ 9.11.13 were determined. The application of these results to the study of the Momordica balsamina seed oil shows that this oil contains two conjugated octadecatrienoic fatty acids in appreciable amounts, punicic acid (50%) and α‐eleostearic acid (13%). The isomerization of conjugated acids in M. balsamina seed oil was followed for one year. Quantitation of octadecatrienoic acids using GLC gave results similar to those obtained with 13 C NMR.