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Polyethylene glycol modification of Candida rugosa lipase
Author(s) -
Baillargeon Mary Welch,
Sonnet Philip E.
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542388
Subject(s) - candida rugosa , lipase , polyethylene glycol , peg ratio , chemistry , hydrolysis , oleic acid , organic chemistry , triacylglycerol lipase , stearic acid , polymer chemistry , biochemistry , enzyme , finance , economics
Polyethylene glycol (PEG) was covalently attached to lipase (EC 3.1.1.3) from Candida rugosa yielding a modified lipase of higher specific activity in hydrolytic and synthetic reactions in organic solvents. PEG of molecular weights 5000 and 1900 solubilized the lipase in selected organic solvents, but PEG of molecular weight 750 was too small to accomplish this completely. The modified lipase was 10 times more stable in water than native lipase, but was less stable in benzene. The selectivity of the modified lipase was also altered to favor reaction with oleic versus stearic acid.