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The determination of fatty amides by high performance liquid chromatography
Author(s) -
Jasperse J. L.
Publication year - 1988
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542386
Subject(s) - chromatography , high performance liquid chromatography , chemistry , chloroform , fatty acid , solvent , acetic acid , hexane , nitrile , amide , silica gel , organic chemistry
A high pressure liquid chromatographic technique for separating fatty amides by chain length in the presence of fatty nitriles was developed. The separation used spherical silica with hexane/chloroform/glacial acetic acid (7:2:1, v/v/v) as the mobile phase. The HPLC method can be used to detect trace amounts of fatty amide in the presence of fatty nitrile with a recovery of 99%. Thin layer chromatography was used as a solvent scanning technique. The relationship between k values and R f values was investigated.