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Lipid‐lipase interactions. 2. A new method for the assay of lipase activity
Author(s) -
Linfield Warner M.,
Serota Samuel,
Sivieri Lorraine
Publication year - 1985
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542312
Subject(s) - lipase , rhizopus arrhizus , candida rugosa , sonication , hydrolysis , chromatography , aspergillus niger , olive oil , chemistry , substrate (aquarium) , triacylglycerol lipase , organic chemistry , biochemistry , enzyme , food science , biology , ecology
A simplified procedure for the measurement of activity was developed for lipases (EC 3.1.1.3) from Chromobacterium viscosum, Candida rugosa, Aspergillus niger and Rhizopus arrhizus . It differs from existing procedures in that olive oil, refined with a bleaching clay, was used as the substrate, periodic sonication was applied to promote efficient emulsification, the hydrolysis time was extended to 1 hr, and no additive such as protective colloids and surfactants were used. The activity was determined arbitrarily for that amount of lipase required to bring about 24% hydrolysis/hr at room temperature Unlike currently used assay procedures, the present method gives results which are highly reproducible with a maximum standard deviation of 2.5% and, more commonly, less than 1.00%.