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Recovery of eicosapentaenoic acid from fungal mycelia by solvent extraction
Author(s) -
O’Brien Dennis J.,
Senske Gerard E.
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542259
Subject(s) - extraction (chemistry) , solvent , mycelium , eicosapentaenoic acid , chromatography , chemistry , hexane , solvent extraction , microorganism , fatty acid , organic chemistry , botany , biology , bacteria , polyunsaturated fatty acid , genetics
Utilization of lipids containing eicosapentaenoic acid (EPA) produced by microorganisms requires processes for their efficient recovery from microbial cells. Recovery of EPA from mycelia of the fungus Pythium irregulare by solvent extraction with hexane‐isopropanol (HIP) in a pilot‐plant colloid mill was investigated. Extraction efficiencies of 96% for lipid and EPA were achieved with a 3∶2 (vol/vol) HIP mixture by milling wet, filtered mycelia for 5 min at a solvent/dry solids ratio of 100 L/kg. The process yielded a crude extract that contained up to 96% lipid and an EPA content as high as 24% (with no selectivity for EPA).