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A comparison of high‐performance liquid chromatography and spectrophotometry to measure chlorophyll in canola seed and oil
Author(s) -
Ward Kerry,
Scarth Rachael,
Daun J. K.,
Thorsteinson C. T.
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02542256
Subject(s) - canola , chlorophyll , high performance liquid chromatography , chromatography , chlorophyll a , chemistry , absorption (acoustics) , chlorophyll b , pigment , spectrophotometry , materials science , food science , biochemistry , organic chemistry , composite material
There are several methods available to measure chlorophyll in canola oil and seed, and these will not necessarily yield the same results and should not be used in terchangeably. Total chlorophyll was determined for samples of canola seed and commercial canola oil by recognized spectrophotometric methods and by high‐performance liquid chromatography (HPLC). The HPLC method, which summed all chlorophyll‐related pigments detected, found approximately 1.4 times more total chlorophyll per sample than did the spectrophotometric methods. The spectrophotometric methods are calibrated with only chlorophyll a and underestimate other chlorophyll pigments, which have lower extinction, coefficients and different absorption maxima. The HPLC method detects each pigment at its absorption maxima and applies the appropriate absorptivity factor. Care must be taken when comparing results obtained by different methods. There appears to be a need for a standardized method of chlorophyll pigment measurement by HPLC.