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The isolation of omega‐3 polyunsaturated fatty acids and methyl esters of fish oils by silver resin chromatography
Author(s) -
Adlof R. O.,
Emken E. A.
Publication year - 1985
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541694
Subject(s) - polyunsaturated fatty acid , acetonitrile , chemistry , fish oil , acetone , chromatography , menhaden , methanol , solvent , elution , high performance liquid chromatography , silver nitrate , organic chemistry , fatty acid , fish <actinopterygii> , biology , fishery
Multigram quantities of the highly unsaturated ω3 component from samples of fish oil fatty acids and esters were isolated by silver resin chromatography. An XN1010 resin column saturated with silver ions was utilized. Polyunsaturated fatty acid (PUFA) esters from fish oil concentrate (FOC) were fractionated based on the number of double bonds by using solvent programming (acetonitrile in methanol). Larger samples (4–9 g) of FOC acids and esters and menhaden acids and esters were enriched in ω3 polyunsaturates to 82–99% (95–99% total PUFA) by use of a large 100% silver resin column and isocratic elution with 30, 35 or 45% acetonitrile in acetone.