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Lipid methodology — Chromatography and beyond. Part I. GC/MS and LC/MS of glycerolipids
Author(s) -
Kuksis A.,
Myher J. J.,
Marai L.
Publication year - 1984
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541637
Subject(s) - chromatography , mass spectrometry , chemistry , high performance liquid chromatography , resolution (logic) , chemical ionization , glyceride , electron ionization , direct electron ionization liquid chromatography–mass spectrometry interface , gas chromatography , liquid chromatography–mass spectrometry , analytical chemistry (journal) , ionization , ion , fatty acid , organic chemistry , artificial intelligence , computer science
This paper reviews recent examples of the application of combined high temperature gas‐liquid chromatography (GLC) and reversed phase high pressure liquid chromatography (HPLC) with electron impact and chemical ionization mass spectrometry for structural studies of natural diacyl and triacylglycerols. It was concluded that the combination of reversed phase HPLC with direct liquid inlet chemical ionization mass spectrometry provides the most complete resolution and most reliable identification of natural acylglycerols, far exceeding the capabilities of either technique alone. The LC/MS method is suitable for quantitative analysis following appropriate calibration of the total or fragment ion response.