Detection of chlorophyll derivatives in soybean oil by HPLC | Zendy

Fraser M. S. | Zendy; Frankl G. | Zendy

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Detection of chlorophyll derivatives in soybean oil by HPLC

Author(s) -

Fraser M. S.,

Frankl G.

Publication year - 1985

Publication title -

journal of the american oil chemists' society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.512

H-Index - 117

eISSN - 1558-9331

pISSN - 0003-021X

DOI - 10.1007/bf02541506

Subject(s) - pheophytin , high performance liquid chromatography , chromatography , chemistry , acetone , pigment , methanol , chlorophyll a , chlorophyll , organic chemistry , biochemistry , photosynthesis , photosystem ii

Chlorophyll derivatives have been isolated from a degummed soybean oil by cellulose column chromatography and resolved by reversed phase HPLC. The HPLC separation was performed on a Zorbax ODS column using acetone‐methanol (75:25) as the mobile phase. Seven major components were detected by visible (650 nm) light absorption. Pheophytin A is the predominant component of the mixture (40–45% of the total). Pheophytin A’, pyropheophytin A, and three unidentified pigments having spectral features resembling pheophytin A comprise the other major pigments. No evidence was found for the presence of chlorophylls A and B in this oil.

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