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Comparative studies of three solvent mixtures for the extraction of soybean lipids
Author(s) -
Khor H. T.,
Chan S. L.
Publication year - 1985
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541501
Subject(s) - methanol , chemistry , chromatography , chloroform , hexane , extraction (chemistry) , methylene , thin layer chromatography , chloride , solvent , silica gel , gas chromatography , column chromatography , charring , organic chemistry
Soybean seeds were extracted with chloroform‐methanol (2:1), methylene chloride‐methanol (2:1) and hexane‐isopropanol (3:2) mixtures. The seed lipids were then fractionated by column chromatography. Neutral lipids were further separated by thin layer chromatography (TLC) and quantified by acid charring method. Fatty acid methyl esters were prepared and analyzed by gas liquid chromatography (GLC). Our results show that methylene chloride‐methanol (2:1) was as good as chloroform‐methanol (2:1) for the extraction of soybean lipids, but hexane‐isopropanol (3:2) was somewhat inferior.