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Influence of surfactants on an extracellular lipase from Pythium ultimum
Author(s) -
Mozaffar Z.,
Weete J. D.,
Dute R.
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541475
Subject(s) - pythium ultimum , lipase , chaps , pulmonary surfactant , taurocholic acid , emulsion , chemistry , triton x 100 , chromatography , extracellular , enzyme , chemical engineering , biochemistry , biology , botany , bile acid , biological pest control , engineering
Abstract The activity of an extracellular lipase from the fungus Pythium ultimum was studied as a function of emulsion properties, and the amount and type of surfactants used in preparing the emulsions. The highest emulsified globule surface area as a function of surfactant concentration and emulsion stability were in the order taurocholic acid >Triton X‐100>3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS). Lipase activity was dependent on both the type and the concentration of surfactant, with taurocholic acid and CHAPS giving tenfold stimulation over the control at 0.1 and 0.8 mM, respectively. The evidence suggests that the influence of surfactant is related more to interaction with the enzyme at the oil‐water interface than to providing greater interfacial surface area.