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Extraction partition and column chromatographic separation of aflatoxins B 1 and M 1
Author(s) -
Masri Merle Sid
Publication year - 1970
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541459
Subject(s) - aflatoxin , chromatography , chemistry , elution , extraction (chemistry) , aqueous solution , solvent , chloroform , silica gel , organic chemistry , food science
Extraction, partition and chromatographic elution characteristics of aflatoxin were studied. The novel use of aqueous dimethylsulfoxide or aqueous dimethylformamide for extraction from agricultural products was tested and found effective. Partition studies suggest advantages in analytical work of using solvent pairs in which benzene rather than (the usually employed) chloroform is used to transfer aflatoxin B 1 from primary extracts (aqueous phases). Tests of elution characteristics of aflatoxins B 1 and M 1 on silica gel columns with different developing solvents provided a basis for a procedure in which aflatoxins B 1 and M 1 of a test sample are recovered in separate eluates in which substances which interfere with TLC separation are minimized.