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The bioassay of aflatoxins and related substances with Bacillus megaterium spores and chick embryos
Author(s) -
Jayaraman A.,
Herbst E. J.,
Ikawa M.
Publication year - 1968
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541261
Subject(s) - aflatoxin , bioassay , bacillus megaterium , spore , embryo , biology , incubator , food science , microbiology and biotechnology , incubation , bacteria , biochemistry , genetics
The “paper disc plate” method of antibiotic assay using Bacillus megaterium spores was tested for the assay of aflatoxins and related compounds. Significant inhibitions were obtained in the range of 1–4 µg/disc for aflatoxin B 1 and 4–8 µg/disc for aflatoxin G 1 . Dicumarol was the most active of the compounds tested and inhibited at 0.1–1.0 µg/disc. Compounds were also tested using nine‐day‐old chick embryos. It was found that embryos incubated in egg cartons were much more sensitive to toxins than eggs incubated on cotton padding or in a commercial egg incubator. The LD 100 of aflatoxin B 1 to carton‐incubated embryos was 0.01 µg, while to embryos from the commercial incubator the LD 100 was >5.0 µg. The results of the two bioassay procedures did not correlate completely with each other.