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Aflatoxins B 1 , B 2 , G 1 , and G 2 : Separation and purification
Author(s) -
Stubblefield R. D.,
Shotwell O. L.,
Shan G. M.
Publication year - 1968
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541258
Subject(s) - silicic acid , chloroform , chemistry , silica gel , chromatography , aflatoxin , acetone , ethanol , methanol , column chromatography , thin layer chromatography , hexane , pigment , organic chemistry , food science
Aflatoxins B 1 , B 2 , G 1 , and G 2 have been separated on a series of chromatographic columns. Chromatography of crude products isolated from molded wheat and rice on silicic acid with washed chloroform:ethanol (99:1) gave relatively pure B 1 . The rest of the column fractions containing predominantly G 1 , along with B 1 , B 2 , and G 2 , were pooled and fractionated on a Silica Gel G column. The mobile phase was washed chloroform:acetone:ethanol (97.3:2.0:0.75). Thin‐layer chromatography was used to follow column development. Each of the aflatoxins was treated with either decolorizing carbon or copper carbonate to remove colored pigments, and rechromatographed on Silica Gel G. Crystalline aflatoxins were prepared from chloroform solutions by addition of n ‐hexane, methanol, or ethanol.