Premium
A simplified procedure for the determination of aflatoxin B 1 in cottonseed meals
Author(s) -
Velasco James
Publication year - 1969
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541219
Subject(s) - aflatoxin , densitometer , chromatography , cottonseed , cottonseed meal , reagent , chemistry , food science , organic chemistry , bran , physics , raw material , quantum mechanics
A simplified procedure for the detection of aflatoxin B 1 in cottonseed meals has been developed. The procedure substantially reduces the time and cost of aflatoxin analysis. A single chromatographic column of celite is used to concentrate and purify the aflatoxin fractions. A new solvent system of ether‐methanol‐water (96:3:1) improves separation of aflatoxin fractions on TLC plates. This improvement permits measurement of B 1 spots in one sweep of the plate with a densitometer using a 10–15 mm slit. The number of samples which can be screened for aflatoxin contamination on one TLC plate is doubled by re‐use of the plate. The use of technical reagents and stainless steel beakers also helps reduce the time and cost of analysis. The procedure is sensitive to about 5 µg of B 1 per kg of meal.