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Diffuse reflectance fourier transform infrared spectroscopy of phospholipid adsorption onto silicic acid
Author(s) -
Adhikari C.,
Proctor A.,
Blyholder G. D.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541093
Subject(s) - silicic acid , adsorption , chemistry , fourier transform infrared spectroscopy , silanol , hydrolysis , infrared spectroscopy , attenuated total reflection , phosphate , infrared , diffuse reflectance infrared fourier transform , inorganic chemistry , organic chemistry , chemical engineering , catalysis , photocatalysis , physics , optics , engineering
Diffuse reflectance Fourier transform infrared spectroscopy was used to study the mode of adsorption of phosphatidylcholine (PC) in hexane onto silicic acid (SA). PC adsorption was mainly through the charged phosphate group with minimal binding through the ester carbonyl. When the SA surface with adsorbed PC is washed with hexane, containing a small concentration of isopropanol, the desorbed PC is recovered without structural change, i.e., there is no evidence of PC hydrolysis in the adsorption process. Adsorbent misture probably promotes PC adsorption due to the increased availability of surface water hydroxyl groups for interaction with the PC phosphate groups. Isopropanol promoted PC binding by destabilizing PC reverse miscelles in solution, thus promoting its adsorption.