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Characterization of brighteners in detergents by high‐performance liquid chromatography/mass spectrometry/ultraviolet/fluorescence and three‐dimensional high‐performance liquid chromatography
Author(s) -
Ogura I.,
DuVal D. L.,
Miyajima K.
Publication year - 1995
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02541033
Subject(s) - chromatography , high performance liquid chromatography , mass spectrometry , chemistry , ultraviolet , chromatography detector , fluorescence , fluorescence spectroscopy , materials science , physics , optoelectronics , quantum mechanics
A method has been developed for the identification of unknown brighteners in detergents by a high‐performance liquid chromatography (HPLC)/mass spectrometry (MS)/ultraviolet (UV)/fluorescence system with MS/MS capability. Ten brighteners from five groups—stilbene, biphenyl stilbene, pyrazoline, oxazole, and coumarin derivatives—were separated on a C 8 reverse‐phase HPLC column. The UV and fluorescence detectors positively located the brightener peaks and differentiated between trans and cis isomers separated by the chromatographic system. Ion‐spray (IS)/MS and IS/MS/MS spectra were then used to identify the structure of unknown brighteners in detergent products. An HPLC with a diode‐array detector provided a quick identification check and accurate quantitative data for known brighteners, even on overlapping peaks.

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