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Incorporation of [1‐ 14 C]octadecanol into the lipids of Leishmania donovani
Author(s) -
Herrmann H.,
Gercken G.
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02540966
Subject(s) - ether , chemistry , plasmalogen , phosphatidylethanolamine , alkyl , lipophilicity , alkoxy group , moiety , vinyl ether , stearate , side chain , leishmania donovani , organic chemistry , stereochemistry , phospholipid , biochemistry , phosphatidylcholine , membrane , leishmania , polymer , copolymer , parasite hosting , world wide web , computer science
After incubation of stationary phase Leishmania donovani with [1‐ 14 C] octadecanol, about 70% of the precursor was taken up within 3 hr. Wax esters and acyl moieties of glycerolipids contained most of the 14 C‐activity from 3 to 6 hr, because octadecanol was partly oxidized to stearate. Ether moieties were only weakly labeled. After 40 hr, 1‐0‐aklyl and 1‐0‐alk‐1′‐enyl diacylglycerols as well as 1‐0‐alkyl and 1‐0‐alk‐1′‐enyl‐2‐acyl‐ sn ‐glycero‐3‐phosphoethanolamines contained nearly all of the radioactivity. Most of the label in the neutral ether lipids was located in the alkyl ether side chain, whereas, in the phosphatidylethanolamine fraction, most of the label was found in the alkenyl ether side chain. Administration of 1‐0‐[1‐ 14 C] hexadecyl glycerol resulted in rapid labeling of the vinyl ether side chain of phosphatidylethanolamine plasmalogen (1 hr) increasing further at 2.5 hr. Most of the radioactivity in the alkoxy diacylglycerols was found in the 1‐0‐alkyl moiety.