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Inhibition of mitochondrial fatty acid elongation by antibodies to 3‐ketoacyl‐CoA thiolase
Author(s) -
Staack Harold,
Davidson Bruce,
Schulz Horst
Publication year - 1980
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02540965
Subject(s) - thiolase , elongation , biochemistry , lipidology , limiting , fatty acid , acetone , chemistry , mitochondrion , clinical chemistry , enzyme , dehydrogenase , mechanical engineering , materials science , engineering , metallurgy , ultimate tensile strength
Antibodies to pig heart 3‐ketoacyl‐CoA thiolase inhibited almost completely and in a parallel fashion thiolase and the acetyl‐CoA‐dependent fatty acid elongation system present in an acetone powder extract of pig heart mitochondria. This finding leads to the conclusion that mitochondrial fatty acid elongation occurs by reversal of fatty acid oxidation. Several lines of evidence point to the thiolase‐catalyzed condensation reaction as the rate‐limiting step in the formation of elongated products. However, the accumulation of hydroxy acids suggests the enoyl‐CoA reductase activity is limiting in the synthesis of saturated fatty acids.