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HPLC analysis of phospholipids by evaporative laser light‐scattering detection
Author(s) -
Mounts T. L.,
Abidl S. L.,
Rennick K. A.
Publication year - 1992
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540944
Subject(s) - high performance liquid chromatography , chromatography , chromatography detector , chemistry , phosphatidylethanolamine , phosphatidylcholine , elution , phospholipid , membrane , biochemistry
High‐performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light‐scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soybeans subjected to storage at high moisture content. Analytical and preparative separations of phospholipids by normal‐phase HPLC were accomplished. Major class fractions were analyzed by transmethylation and capillary collumn chromatography for fatty acid composition, and by reverse‐phase C‐18 HPLC (RP‐HPLC) for molecular species composition. The RP‐HPLC‐ELSD system was limited to the analysis of phosphatidylcholine and phosphatidylethanolamine.