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Separation and quantitative determination of monoacylglycerol mixtures by reversed phase HPLC
Author(s) -
Maruyama Kazushige,
Yonese Chizuo
Publication year - 1986
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540924
Subject(s) - acetonitrile , chromatography , diethyl ether , monoacylglycerol lipase , chemistry , high performance liquid chromatography , phase (matter) , solvent , analytical chemistry (journal) , chloroform , relative standard deviation , quantitative analysis (chemistry) , reversed phase chromatography , coefficient of variation , detection limit , organic chemistry , biochemistry , endocannabinoid system , receptor
The separation and quantitative determination of underivatized saturated and unsaturated monoacylglycerol were examined by reversed phase high performance liquid chromatography using acetonitrile/water as the mobile phase. Monoacylglycerol (MG) mixtures containing saturated MG (C 6 ‐MG‐C 18 ‐MG), monooleoylglycerol and monolinoleoylglycerol were sufficiently resolved, but mixtures of 1‐MG and 2‐MG with the same acyl group could not be separated under the conditions given below: column, C‐8 chemical bonded column (4.6 × 250 mm); sample solvent, diethyl ether (UV detector at 210–215 nm) or chloroform; mobile phase, acetonitrile/water (7:3, v/v). Quantitative determinations were made for mixtures of three components (representative standard MGs) on a reversed phase column (Unisil Q, C‐8) using a UV detector and the internal standard method. Standard deviation and coefficient of variation gave accurate, reproducible results.