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Chromatographic separation of glucopyranosyl sinapate from canola meal
Author(s) -
Amarowicz Ryszard,
Shahidi Fereidoon
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540674
Subject(s) - chromatography , chemistry , fraction (chemistry) , sephadex , solvent , acetic acid , methanol , canola , acetonitrile , high performance liquid chromatography , glycoside , organic chemistry , food science , enzyme
The compound 1‐ O ‐β‐D glucopyranosyl sinapate (GPS), a phenolic glycoside, was separated from ethanolic extracts of defatted canola meal by a two‐step chromatographic method. The first step involved Sephadex LH‐20 chromatography with methanol as the eluting solvent. The solvent from the fraction containing GPS was evaporated, and glucopyranosyl sinapate was subsequently separated by a semi‐preparative high‐performance liquid chromatography method with an RP‐18 column and a mobile phase consisting of water/acetonitrile/acetic acid (88∶10∶2, vol/vol/vol).