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9( Z )‐Octadecenamide and fatty amides by Bacillus megaterium (B‐3437) conversion of oleic acid
Author(s) -
Kaneshiro T.,
Vesonder R. F.,
Peterson R. E.,
Weisleder D.,
Bagby M. O.
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540659
Subject(s) - bioconversion , oleic acid , bacillus megaterium , chemistry , chromatography , mass spectrometry , fatty acid , organic chemistry , catalysis , biochemistry , bacteria , biology , fermentation , genetics
9( Z )‐Octadecenamide, hexadecenamide, tetradecenamide and tetradecanamide were produced by a novel bioconversion of oleic acid with Bacillus megaterium NRRL B‐3437. Although chemical synthesis is more practical, the bioconversion to fatty amides (5–7% of total recovered lipids) was unique for its requirement of both enzymatic catalysis and equimolar oleic acid/ammonium salt substrates. Purified octadecenamide was obtained by silica gel and high‐pressure liquid chromatographic procedures and was characterized by gas chromatography, mass spectrometry, infrared and nuclear magnetic resonance.