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Inhibition of aflatoxin production in Aspergillus flavus infected cotton bolls after treatment with neem ( Azadirachta indica ) leaf extracts
Author(s) -
Zeringue Hampden J.,
Bhatnagar Deepak
Publication year - 1990
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540646
Subject(s) - aflatoxin , locule , aspergillus flavus , azadirachta , biology , spore , mycotoxin , meliaceae , inoculation , horticulture , botany , aqueous suspension , chemistry , food science , aqueous solution , pollen , stamen
In separate treatments, a spore suspension of A. flavus (control), an aqueous leaf extract of the subtropical neem tree plus a spore suspension of A. flavus , or an aqueous neem leaf extract followed by an A. flavus spore suspension were injected 48 hr later onto the surfaces of locks of developing cotton bolls (30‐day post anthesis). Thirteen days after the treatments, the seeds from the locules were harvested and both fungal growth and aflatoxin production were determined. Fungal growth was unaffected by the treatments but the seeds from locules receiving both neem leaf extracts and A. flavus simultaneously exhibited 16% inhibition of aflatoxin production, while the seeds in locules receiving A. flavus spores 48 hr after neem extract was added exhibited >98% inhibition in aflatoxin production. Neem leaf extracts contain an aflatoxin inhibiting factor, however, the neem leaf extract may need to translocate from the fibrous locule surface to the seed, prior to the fungal inoculation, for maximal effect.