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Improved ozonolysis method for analysis of total n−3 fatty acids during hydrogenation of fish oils
Author(s) -
Jacobsen Laila S.,
Jart Aage
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540596
Subject(s) - ozonolysis , iodine value , fish oil , chemistry , double bond , hexane , fish <actinopterygii> , capillary gas chromatography , iodine , chromatography , organic chemistry , phosphine , gas chromatography , catalysis , biology , fishery
A rapid and precise method for determining the total amount of n−3 fatty acids in small samples of fish oil is presented. The oil is ozonized at −10°C in hexane solution, and the ozonides are subsequently reduced with triphenyl‐phosphine at 40°C. The propanal formed is quantitated by capillary gas chromatography. The method was utilized to follow the industrial hydrogenation of a fish oil, and it was demonstrated that the n−3 double bonds were simultaneously reduced to one‐sixth as the iodine value decreased to one‐half.