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Separation of monoacylglycerols by high‐performance liquid chromatography on nitrile‐bonded phase
Author(s) -
Takagi Toru,
Ando Yasuhiro
Publication year - 1994
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540532
Subject(s) - nitrile , monoacylglycerol lipase , chemistry , chromatography , stereospecificity , high performance liquid chromatography , phase (matter) , organic chemistry , catalysis , biochemistry , endocannabinoid system , receptor
Monoacylglycerol molecular species, as their di‐3,5‐dinitrophenylurethane derivatives, were well separated by normalphase high‐performance liquid chromatography on nitrilebonded phase. The peaks emerged in the order 20∶0, 18∶0, 16∶0, 18∶1, 16∶1, 18∶2, and 18∶3. The peaks of 1‐ and 2‐monoacylglycerols with the same acyl group showed complete overlapping. This method could be applied to get acyl compositions in the three positions of triacyl‐ sn ‐glycerols in their stereospecific analysis.