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Increase in the γ‐linolenic acid content by solvent winterization of fungal oil extracted from Mortierella genus
Author(s) -
Yokochi T.,
Usita M. T.,
Kamisaka Y.,
Nakahara T.,
Suzuki O.
Publication year - 1990
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540504
Subject(s) - acetone , glyceride , chemistry , petroleum ether , solvent , chromatography , hexane , extraction (chemistry) , fraction (chemistry) , fatty acid , ether , linolenic acid , organic chemistry , linoleic acid
The fungal oil extracted from Mortierella ramanniana var. angulispora (IFO 8187) was solvent winterized in order to raise the content of γ‐linolenic acid (GLA). Effects of winterization conditions (solvent, oil concentration in the solvent and temperature) and changes of glyceride compositions were discussed. The fungal oil was separated into four diglycerides and 17 triglycerides (TG) with high performance liquid chromatography. The predominant species were POO, POP and LOP, whose contents were 24.4, 22.9 and 9.4% of the total TG, respectively. Ethanol at 4°C gave the highest GLA content of 10.5% in spite of lower yield than with acetone at −20°C. The highest separation efficiency for GLA (η GLA ) was 0.27 with acetone at −20°C and 10% oil concentration, resulting in 8.3% of GLA from the fungal oil at 5.7% LGA. In case of lower oil concentration at 5–20%, η GLA showed higher in the following order: acetone (−20°C)> n ‐hexane (−20°C)>acetone (4°C)>petroleum ether (−20°C). The winterization process also proved to be effective for the separation of TG type, Sa 2 U (Sa; saturated fatty acid; U, unsaturated fatty acid) into the crystallized fraction and SaU 2 into the liquid fraction. Acetone at −20°C showed higher separation efficiency for triunsaturated TG than the other solvents.