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The influence of water on the synthesis of n‐butyl oleate by immobilized Mucor miehei lipase
Author(s) -
Leitgeb M.,
Knez Ž.
Publication year - 1990
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540490
Subject(s) - lipase , chemistry , bar (unit) , water activity , chromatography , mucor , rhizomucor miehei , reaction rate , triacylglycerol lipase , enzyme , organic chemistry , catalysis , water content , food science , geology , geotechnical engineering , penicillium , oceanography
The influence of initial water concentration on the synthesis of n‐butyl oleate was investigated. The synthesis was done with immobilized Mucor miehei lipase—Lypozyme ™ —at various reaction conditions. The activity of the enzyme is lower at higher amounts of water. Initial reaction rates, as well as equilibrium conversion, increase at low initial water concentration. Optimal water concentration for the activity of immobilized lipase is temperature dependent at the pressure of 1 bar. Low initial water concentration barely effects equilibrium esterification at 0.032 bar. At high initial water concentrations equilibrium conversion, as well as initial reaction rates, decrease at both pressures.