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An improved separation of aflatoxins
Author(s) -
Wiley Mabry,
Waiss A. C.
Publication year - 1968
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540173
Subject(s) - aflatoxin , chromatography , silica gel , chloroform , sephadex , chemistry , elution , column chromatography , ethyl acetate , organic chemistry , food science , enzyme
Crude aflatoxin from a chloroform extract of Aspergillus flavus cultures on rice was precipitated with Skelly Solve B and chromatographed on 100–200 mesh silica gel columns, using ethyl acetate as eluant. On this column there was no separation of aflatoxins from each other, but most of the brown, oily material was removed. The next step in the purification was chromatography on 100–200 mesh silica gel columns with chloroform and 5% methanol/chloroform as eluants. A large part of the B 1 was purified, but B 2 , G 1 and G 2 did not separate, and M 1 had a brown oil that prevented crystallization. The M 1 was purified by chromatography on Sephadex LH‐20 with chloroform; the brown material was retained while the M 1 passed through. The separation of aflatoxin B 2 , G 1 , and G 2 was achieved by column chromatography on Silica Gel H for TLC. In addition, aspertoxin was separated and identified. The purity and identity of the compounds were established by 100 MHz NMR.