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Human platelet lipids and their relationship to blood coagulation
Author(s) -
Marcus Aaron J.,
ZuckerFranklin Dorothea
Publication year - 1965
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540091
Subject(s) - silicic acid , platelet , coagulation , chemistry , biochemistry , extraction (chemistry) , chromatography , membrane , lipoprotein , biology , cholesterol , organic chemistry , immunology , medicine
There is now reasonable agreement on the sequence of physiological and biochemical events leading to fibrin formation, and phospholipids are an important part of this process. The phosphatides are ordinarily provided by platelets, and it appears that a lipoprotein complex is responsible for this activity. The anatomic site of this complex is not known, but evidence is presented that it may be a property of the platelet membrane. Methods for the study of platelet lipids including fatty acids and aldehydes are described, and include silicic acid column and paper chromatography, as well as thin‐layer and gas‐liquid chromatographic procedures. These are also being utilized in studies of subcellular platelet particles, where only limited amts of biological material are available for study. It is stressed that experimental results obtained from studies on isolated lipids should be interpreted with a certain degree of caution. It is unlikely that they are available as such in in vivo coagulation, and the drastic procedures used for their extraction and isolation may alter their basic physiological properties.