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Assay of aflatoxin in peanuts and peanut products using acetone‐hexane‐water for extraction
Author(s) -
Robertson J. A.,
Lee Louise S.,
Cucullu Alva F.,
Goldblatt L. A.
Publication year - 1965
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540086
Subject(s) - aflatoxin , chromatography , chemistry , chloroform , extraction (chemistry) , acetone , hexane , solvent , ethyl acetate , aqueous solution , food science , organic chemistry
A quantitative method is described for the assay of aflatoxin in peanut products. The procedure involves extraction of aflatoxin from the sample with a homogeneous acetone‐hexane‐water solvent mixture followed by purification of the extract by phasic extraction of the aflatoxin with aqueous sodium chloride and then with chloroform. The purified chloroform extract is analyzed by thin‐layer chromatography by comparison of the intensity of fluorescence of any aflatoxin with the intensity of a known standard. The aflatoxin analyses of peanuts were found to be very variable due to sampling, and this variability has been greatly reduced by finely grinding and thoroughly mixing 2 kg of the sample before removal of an aliquot for assay. The method is sensitive to approximately 2 parts per billion.