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Effects of double bond configuration on lecithin synthesis
Author(s) -
Lands W. E. M.
Publication year - 1965
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/bf02540085
Subject(s) - lecithin , chemistry , enzyme , cis–trans isomerism , stereochemistry , chromatography , organic chemistry
Stearyl‐CoA, oleyl‐CoA and elaidyl‐CoA were prepared and tested as substances in synthesizing lecithin from 1‐ and 2‐acyglycerol‐3‐phosphorylcholine. The rates of acyltransfer were followed by a continuous spectrophotometric assay. The enzyme(s) used to catalyze esterification at the 2‐position did not discriminate appreciably between the cis and trans isomers of octadecenoate and transferred them more rapidly than the saturated acid, octadecanoate. However, the enzyme(s) which acted at the 1‐position discriminated markedly between the geometric isomers and rapidly transferred to trans ‐ octadecenoate and octadecanoate.