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Preparation of tritium‐labeled sterols and the synthesis of labeled‐24‐azacholesterol
Author(s) -
Thompson Malcolm J.,
Berngruber Otto W.,
Klein Peter D.
Publication year - 1971
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02538393
Subject(s) - campesterol , tritium , stigmasterol , chemistry , chromatography , radiochemistry , lipidology , sterol , clinical chemistry , biochemistry , cholesterol , physics , nuclear physics
A method is presented for the preparation of 2,4‐ 3 H‐sterols (stigmasterol, β‐sitosterol, campesterol, fucosterol and 7‐dehydrocholesterol) via the technique of exchange‐labeling of keto steroids and their subsequent conversion to sterols. The preparation of tritium‐labeled 3β‐hydroxy‐24‐norchol‐5‐en‐23‐oic acid and the synthesis of 2,4‐ 3 H‐24‐azacholesterol are reported. Some advantages of the exchange‐labeling method over the conventional catalytic exchange method are discussed.