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Enantiomer separations of mixtures of monoacylglycerol derivatives by HPLC on a chiral column
Author(s) -
Takagi Toru,
Ando Yasuhiro
Publication year - 1990
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537983
Subject(s) - enantiomer , chemistry , chiral column chromatography , chromatography , monoacylglycerol lipase , homologous series , high performance liquid chromatography , chiral derivatizing agent , organic chemistry , silica gel , endocannabinoid system , receptor , biochemistry
High‐performance liquid chromatographic separations of monoacylglycerol enantiomeric mixtures as their di‐3,5‐dinitrophenylurethane derivatives were carried out on a chiral stationary phase, N‐( R )‐1‐(α‐naphthyl)ethylaminocarbonyl‐( S )‐valine bonded to silica gel. The conditions for the quantitative analysis of sn ‐1 and sn ‐3 enantiomeric mixtures of six saturated 1‐monoacylglycerols having consecutive even carbon numbers and of sn ‐1 and sn ‐3 enantiomeric mixtures of 1‐stearoyl‐, 1‐oleoyl‐, 1‐linoleoyl‐, and 1‐linolenoyl‐glycerols were obtained. The linear relationships of logarithmic retention volumes against the number of carbons and olefinic bonds for the enantiomer homologous series are discussed, and a new concept of enantiomer separation is presented.