Premium
Microwave‐mediated methanolysis of lipids and activation of thin‐layer chromatographic plates
Author(s) -
Khan Mobashsher U.,
Williams John P.
Publication year - 1993
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537507
Subject(s) - glyceride , chromatography , chemistry , polyunsaturated fatty acid , fatty acid , thin layer chromatography , fraction (chemistry) , thin layer , silica gel , microwave , fatty acid methyl ester , microwave oven , organic chemistry , layer (electronics) , catalysis , biodiesel , physics , quantum mechanics
A technique is described for the methanolysis of fatty acids from acylglycerols with HCl/CH 3 OH or NaOH/CH 3 OH using a microwave oven. The esterification is rapid and complete and does not result in significant degradation of polyunsaturated fatty acids, even in the presence of oxygen. The fatty acid compositions of intact tissues were also determined using this technique. The microwave oven has also been used to condition normal silica gel and argentation thin‐layer chromatographic plates in a fraction of the time normally required.