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Effects of triarimol, tridermorph and triparanol on sterol biosynthesis in carrot, tobacco and soybean suspension cultures
Author(s) -
Hosokawa Glenn,
Patterson Glenn W.,
Lusby William R.
Publication year - 1984
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537407
Subject(s) - stigmasterol , campesterol , sterol , phytosterol , nicotiana tabacum , chemistry , biology , biosynthesis , alkylation , biochemistry , botany , food science , cholesterol , chromatography , gene , catalysis , enzyme
The effects of triarimol, tridemorph and triparanol on sterol biosynthesis in carrot, tobacco and soybean suspension cultures were studied. The 3 plant species normally contain campesterol, stigmasterol and sitosterol as major sterols. Triarimol inhibited demethylation at C 14 and the second alkylation of the side chain in all 3 species. The primary effects of tridemorph were the inhibition of the opening of the 9β,19‐cyclopropane ring and the second alkylation of the side chain. Triparanol treatments resulted in the accumulation of 14α‐methyl sterols, and the inhibition of second alkylation in the side chain in carrot and tobacco cultures. Cyclopropyl sterols also accumulated in carrot and tobacco cultures treated with triparanol. Triparanol did not alter the sterol composition of soybean cultures except for decreasing concentrations of campesterol and stigmasterol and increasing amounts of sitosterol.