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A new quantitative method for the analysis of monoacylglycerol isomers using 13 C nuclear magnetic resonance spectroscopy
Author(s) -
Dawe R. D.,
Wright J. J. C.
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537348
Subject(s) - monoacylglycerol lipase , chemistry , isomerization , regioselectivity , hydrolysis , monoglyceride , nuclear magnetic resonance spectroscopy , chromatography , organic chemistry , catalysis , fatty acid , biochemistry , endocannabinoid system , receptor
Current methods for determining the regiochemistry of monoacylglycerols are lengthy, tedious and aggravated by the ready isomerization of 2‐acyl‐ sn ‐glycerols. A new method employs a very rapid adsorption chromatography step in which isomerization is kept to a minimum, followed by formation of the isopropylidene derivatives using mild procedures. These cyclic derivatives of 1‐ and 3‐acyl‐ sn ‐glycerols and 2‐acyl‐ sn ‐glycerols are stable thermally and display certain characteristic 13 C NMR resonances. Integration of these resonances yields directly the proportion of monoacylglycerol isomers in the mixture. The method was applied to the analysis of monoacylglycerols produced by enzymatic hydrolysis of synthetic and naturally occurring triacylglycerols.