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Measurement of lipid peroxidation in vivo: A comparison of different procedures
Author(s) -
Pompella Alfonso,
Maellaro Emilia,
Casini Alessandro F.,
Ferrali Marco,
Ciccoli Lucia,
Comporti Mario
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537304
Subject(s) - lipid peroxidation , malondialdehyde , chemistry , in vivo , biochemistry , phospholipid , glutathione , bromobenzene , microsome , lipidology , chromatography , clinical chemistry , polyunsaturated fatty acid , in vitro , membrane , antioxidant , fatty acid , biology , enzyme , microbiology and biotechnology , catalysis
A study was undertaken to investigate whether some of the methods commonly used to detect lipid peroxidation of cellular membranes in vivo correlate with each other. The study was performed with the livers of bromobenzene‐intoxicated mice, in which lipid peroxidation develops when the depletion of glutathione (GSH) reaches a threshold value. The methods tested and compared were the following: i) measurement of the malondialdehyde (MDA) content of the liver; ii) detection of diene conjugation absorption in liver phospholipids; iii) measurement of the loss of polyunsaturated fatty acids in liver phospholipids; and iv) determination of carbonyl functions formed in acyl residues of membrane phospholipids as a result of the peroxidative breakdown of phospholipid fatty acids. Correlations among the values obtained with these methods showed high statistical significances, indicating that the procedures measure lipid peroxidation in vivo with comparable reliability. Analogously, the four methods appeared also to correlate when applied to in vitro microsomal lipid peroxidation.