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Electron microscopic cytochemistry of cyclopropenoids in cottonseed ( Gossypium hirsutum L.) tissues
Author(s) -
Yatsu Ly,
Kircher H. W.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537267
Subject(s) - cytochemistry , staining , electron microscope , botany , chemistry , sudan black b , biology , ultrastructure , pathology , genetics , medicine , physics , optics
An electron microscopic cytochemical procedure for locating cyclopropenoids (CYP) in oilseeds is proposed. The procedure is based on the fact that metallic silver is produced when AgNO 3 reacts with CYP. Since so many other compounds react with AgNO 3 , we treated specimens that do and not contain CYP with AgNO 3 and looked for electron‐dense stains that were unique to CYP(+) seeds. Virtually everything in all seed specimens, regardless of the presence or absence of CYP, was stained. However, cottonseeds contained two additional staining constituents not found in controls: (i) certain large, intracellular droplets, and (ii) the cuticles, both occurring exclusively in the axial organs. Extraction of the speciments with ether abolished the stainability of the droplets but did not affect cuticles. Arguments for the validity of the test are presented.