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Fatty acid distribution in the phospholipids of Francisella tularensis
Author(s) -
Anderson Robert,
Rashid Bhatti A.
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537220
Subject(s) - francisella tularensis , lipidology , clinical chemistry , fatty acid , chemistry , microbiology and biotechnology , food science , biochemistry , biology , virulence , gene
Abstract Francisella tularensis , LVS (live vaccine strain) grown in a chemically defined medium was found to have a lipid content of 21% by dry weight. The two major phospholipids were identified as phosphatidylethanolamine (PE; 76%) and phosphatidylglycerol (PG; 24%) by thin layer chromatographic analysis, staining characteristics and quantitative chemical analyses of fatty acid, phosphate and glycerol constituents. PE contained a high proportion of 24∶0 fatty acid, with lesser amounts of 24∶1, 22∶0 and 10∶0. The major fatty acids of PG were 18∶1 and 22∶0. Hydroxy fatty acids, which are prominent components of F. tularensis , were conspicuously lacking in these phospholipids; it is therefore concluded that hydroxy fatty acids are constituents of other structures of the organism.