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Pyrophosphatidic acid in mushrooms
Author(s) -
Sugai Akihiko,
Itoh Toshihiro,
Kaneko Hiroshi,
Kinjo Noriko,
Muramatsu Toshio
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02537219
Subject(s) - silicic acid , chromatography , chemistry , column chromatography , phosphatidic acid , silica gel , thin layer chromatography , mushroom , organic chemistry , biochemistry , food science , phospholipid , membrane
The distribution of pyrophosphatidic acid in mushrooms was investigated. Total lipids of mushrooms were extracted from their fruit body, and the pyrophosphatidic acid fraction was isolated by DEAE‐silica gel column and silicic acid column chromatography. The presence of pyrophosphatidic acid was tested by thin layer chromatography. Of the 43 species of mushrooms examined, the acid was found in 30. The pyrophosphatidic acid was identified by chromatography, IR spectroscopy and quantitative degradation with aqueous pyridine to produce phosphatidic acid. Chemically synthesized pyrophosphatidic acid was used as standard.